Media Preparation Calculator

Calculate reagent volumes for cell culture media batches using C1V1 = C2V2 dilution.

Volume per flask (mL)

Number of flasks

FBS (%)

Antibiotic (% v/v)

L-Glutamine (% v/v)

Other supplement (% v/v)

Tab to move between fields

Total volume needed (mL)

500

Base media (mL)440

FBS volume (mL)50

Antibiotic volume (mL)5

L-Glutamine volume (mL)5

Supplement volume (mL)0

Estimated cost ($)$33.8

How to Use This Calculator

Enter the desired volume per flask (mL) and number of flasks to prepare.
Set FBS (%), antibiotic (%), L-Glutamine (%), and any other supplement percentages.
Review Total Volume Needed (mL), Base Media (mL), FBS Volume (mL), and each supplement volume.
Print the outputs as a preparation checklist to ensure accurate, reproducible media formulations.

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