Protein Concentration Calculator
Protein quantification from Bradford/BCA assay data using Beer-Lambert Law (A = εlc).
Inputs
Results
Concentration (mg/mL)
0
Concentration (µg/mL)0.76
Concentration (µM)11.41
Concentration (M)0
Total protein (mg)0
A280-based conc. (mg/mL)0
How to Use This Calculator
- Enter the absorbance reading from your spectrophotometer and the molar extinction coefficient (ε).
- Set path length (cm), molecular weight (Da), dilution factor, and sample volume (mL).
- Review Concentration in mg/mL, µg/mL, and µM.
- Use Total Protein (mg) to calculate yield across the full sample volume.
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