Viral Titer Calculator

Calculate viral titer (PFU/mL) from plaque assay data and determine volume needed for target MOI.

Total plaque count

Number of replicates

Dilution (10^-n)

Volume plated (mL)

Target MOI

Target cell count

Tab to move between fields

Titer (PFU/mL)

175,000,000

Titer (log10 PFU/mL)8.24

Average plaques/plate17.5

Volume for MOI (µL)5.71

Volume for MOI (mL)0.01

MOI per 1 µL stock0.18

Total Pfu175,000,000

How to Use This Calculator

Enter the total plaque count and number of replicate plates counted.
Set the dilution exponent (10^-n), volume plated per well (mL).
Enter target MOI and cell count for your infection experiment.
Review Titer (PFU/mL), Volume for MOI (µL), and MOI per 1 µL Stock.
Use Volume for MOI to pipette the correct amount of virus stock for your infection.

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