Pipette Calibration Calculator

Verify pipette accuracy and precision using the gravimetric method (ISO 8655) with Z-factor temperature correction.

Nominal volume (μL)

Mass reading 1 (mg)

Mass reading 2 (mg)

Mass reading 3 (mg)

Mass reading 4 (mg)

Mass reading 5 (mg)

Lab temperature (°C)

Tab to move between fields

Overall pass (1=yes, 0=no)

Mean dispensed volume (μL)100.27

Accuracy error (μL)0.27

Accuracy error (%)0.27

CV% (precision)0.21

Standard deviation (μL)0.21

Range (μL)0.5

Z-factor (μL/mg)1

ISO accuracy limit (%)0.8

ISO precision limit (%)0.2

Accuracy pass (1/0)1

Precision pass (1/0)0

Z Factor1

How to Use This Calculator

Enter the pipette's nominal volume setting in microliters.
Input the measured gravimetric weight in milligrams from 10 replicate dispenses.
Set the water temperature for density correction (typically 20-25°C).
Review the calculated mean volume, systematic error (%), and CV% for precision.
If error exceeds ±2% or CV exceeds 1%, send the pipette for recalibration before use.

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