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Calcimator

Serial Dilution Calculator

Calculate final concentration, diluent volume per tube, and total volume needed for serial dilution series in the laboratory.

Inputs

units/mL
x
steps
mL

Results

Final Concentration

0.01 units/mL

Diluent Per Tube

0.89 mL

Total Volume Needed5.45 mL
How to Use This Calculator
  1. Enter the starting stock concentration and dilution factor per step.
  2. Set the number of dilution steps needed for your assay.
  3. Input the volume needed at each dilution step.
  4. Review the concentration at each step and the volume of diluent to add.
  5. Prepare dilutions in order from most concentrated to least concentrated to minimize carry-over error.
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