Hemocytometer Cell Count

Calculate cell concentration (cells/mL) from hemocytometer counts, number of squares, and dilution factor.

Total Cells Counted

Squares Counted

Dilution Factor

Tab to move between fields

Cells/mL

1,000,000

Cells/mL (millions)

1 ×10⁶

Avg Cells per Square50

Log₁₀ Cells/mL6

Total Cells Counted250

How to Use This Calculator

Enter Total Cells Counted, Squares Counted, and Dilution Factor.
Review Cells/mL and Cells/mL (millions) (×10⁶).
Use Avg Cells per Square and Log₁₀ Cells/mL to inform your decision.
Use the chart to visualize the results and explore different scenarios by adjusting inputs.

How the result changes with Total Cells Counted

Total Cells Counted: Total number of cells counted across all squares. Count cells touching the top and left lines but not bottom and right.
Squares Counted: Number of large squares counted on the hemocytometer grid. Typically 4 or 5 corner squares.
Dilution Factor: Dilution factor used (e.g., 2 for a 1:2 dilution with trypan blue).

Cells/mL = (total cells / squares counted) × dilution factor × 10⁴

Identify Input Parameters
Total Cells Counted = 250, Squares Counted = 5, Dilution Factor = 2 = 3 input(s) provided
Calculate Cells/mL
Cells/mL
1000000 = 1000000
Calculate Cells/mL
Cells/mL
1 = 1
Calculate Avg Cells per Square
Avg Cells per Square
50 = 50
Calculate Log₁₀ Cells/mL
Log₁₀ Cells/mL
6 = 6