PCR Primer Calculator
Calculate melting temperature (Tm), GC content, and annealing temperature for PCR primers.
Inputs
Results
Melting Temperature (Tm)
51.8 °C
Recommended Annealing Temp
46.8 °C
GC Content
50%
G + C Count10
A + T Count10
Primer Length20 bp
Molecular Weight6,600 Da
How to Use This Calculator
- Enter Primer Length (bp), G (Guanine) Count, and C (Cytosine) Count.
- Review Melting Temperature (Tm) (°C), Recommended Annealing Temp (°C), and GC Content (%).
- Use G + C Count and A + T Count to inform your decision.
- Use the chart to visualize the results and explore different scenarios by adjusting inputs.
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Formula
Tm = 64.9 + 41 × (G+C − 16.4) / length (for primers > 13 bp)Related Calculators
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