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Calcimator

Purification Yield Calculator

Calculate overall downstream purification yield from individual step recoveries for biologics manufacturing.

Inputs

%
%
%
%
%
%
%

Results

Overall yield (%)

65.34

Final product (g)32.67
Mass lost (g)17.33
After capture (g)45.13
After polishing (g)35.46
After final processing (g)32.67
Start needed for 1g final (g)76.52
How to Use This Calculator
  1. Enter the starting material mass in grams from the bioreactor harvest.
  2. Set yield percentages for each purification step (clarification, capture, viral inactivation, polishing, UF/DF).
  3. Review Overall Yield (%), Final Product (g), and Mass Lost (g).
  4. Identify the step with the greatest loss and optimize to improve overall process yield.

How the result changes with Polish chrom. 1 yield (%)

Polish chrom. 1 yield (%)Overall yield (%)
117.99
3626.14
6547.19
9065.34

What each input means

Starting material (g)
Starting product mass from bioreactor harvest in grams.
Clarification yield (%)
Recovery from harvest clarification (centrifugation/depth filtration). Typical: 90-98%.
Capture chrom. yield (%)
Recovery from capture chromatography (e.g., Protein A). Typical: 90-98%.
Viral inactivation yield (%)
Recovery through viral inactivation step (low pH). Typical: 95-99%.
Polish chrom. 1 yield (%)
Recovery from first polishing chromatography (e.g., IEX). Typical: 85-95%.
Polish chrom. 2 yield (%)
Recovery from second polishing chromatography (e.g., HIC). Typical: 85-95%.
Viral filtration yield (%)
Recovery through nanofiltration viral removal. Typical: 95-99%.
UF/DF yield (%)
Recovery from ultrafiltration/diafiltration. Typical: 90-98%.

What each result means

Overall yield (%)
Cumulative yield across all purification steps.
Final product (g)
Mass of purified product after all steps.
Mass lost (g)
Total product mass lost during purification.
After capture (g)
Product mass after clarification and capture chromatography.
After polishing (g)
Product mass after both polishing chromatography steps.
After final processing (g)
Product mass after all steps including UF/DF.
Start needed for 1g final (g)
Starting material needed to yield the same final mass at current overall yield.

How this is calculated

Worked example, using the default values

  1. Identify Input Parameters
    4 parameters
    Starting material (g) = 50, Clarification yield (%) = 95, Capture chrom. yield (%) = 95, Viral inactivation yield (%) = 97 = 8 input(s) provided
  2. Calculate Overall yield
    Overall yield = overallYield * 100
    65.34 = 65.34
  3. Calculate Final product
    Final product = startingMassG * overallYield
    32.672 = 32.672
  4. Calculate Mass lost
    Mass lost = startingMassG - finalMassG
    17.328 = 17.328

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