Gene Therapy Dose Calculator

Calculate total vector genome dose from patient weight and target vg/kg for AAV and lentiviral gene therapies.

Patient weight (kg)

Dose coefficient

Dose exponent (10^n vg/kg)

Virus yield (vg/mL)

Fill volume per vial (mL)

Manufacturing overfill (%)

Tab to move between fields

Total dose (vg)

700,000,000,000,000

Total dose (log10 vg)14.85

Dose per kg (vg/kg)10,000,000,000,000

Required conc. (vg/mL)700,000,000,000,000

Stock needed (mL)840

Bioreactor runs needed2

How to Use This Calculator

Enter patient weight (kg), dose coefficient, and dose exponent (10^n vg/kg).
Set virus yield (vg/mL), fill volume per vial (mL), and manufacturing overfill (%).
Review Total Dose (vg), Required Concentration (vg/mL), and Stock Needed (mL).
Use Bioreactor Runs Needed to plan manufacturing capacity for clinical or commercial supply.

How the result changes with Dose exponent (10^n vg/kg)

Patient weight (kg): Patient body weight in kilograms.
Dose coefficient: Coefficient of the dose (e.g., 1.1 for 1.1×10^n vg/kg).
Dose exponent (10^n vg/kg): Exponent for dose in vg/kg. Example: 13 = 10^13 vg/kg. Zolgensma uses ~1.1×10^14.
Virus yield (vg/mL): Viral vector yield from manufacturing in vg per mL of harvest.
Fill volume per vial (mL): Volume per vial for final drug product.
Manufacturing overfill (%): Extra manufacturing volume for losses, QC testing, and overfill.

Total dose (vg): Total vector genomes required for this patient.
Total dose (log10 vg): Log10 of total vector genomes for easy comparison.
Dose per kg (vg/kg): Vector genomes per kilogram of body weight.
Required conc. (vg/mL): Required viral concentration in the fill volume.
Stock needed (mL): Total volume of virus stock required including overfill.
Bioreactor runs needed: Estimated number of 500 mL bioreactor runs to produce needed stock.

Identify Input Parameters
4 parameters
Patient weight (kg) = 70, Dose coefficient = 1, Dose exponent (10^n vg/kg) = 13, Virus yield (vg/mL) = 1000000000000 = 6 input(s) provided
Calculate Total dose
Total dose = Number(totalVg.toExponential(2))
700000000000000 = 700000000000000
Calculate Total dose
Total dose = log10(totalVg)
14.85 = 14.85
Calculate Dose per kg
Dose per kg = Number(dosePerKg.toExponential(2))
10000000000000 = 10000000000000